ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (8): 1325-1332.doi: 10.11843/j.issn.0366-6964.2015.08.007

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Genomic Imprinting and Methylation in Regulating Imprinting of Wif1 Gene in Cattle

WU Xi-hong,ZHANG Ming-yue,YANG Wen-zhi,WU Guo-jiang,LI Shi-jie*   

  1. (College of Life Science,Agricultural University of Hebei,Baoding 071000,China)
  • Received:2014-12-22 Online:2015-08-23 Published:2015-08-23

Abstract:

To study the imprinting status and the regulatory mechanism of Wif1 in cattle,the imprinting status of Wif1 gene was analyzed by sequencing method based on a single nucleotide polymorphism (SNP).The results showed that Wif1 gene exhibited monoallelic expressions in lung tissues,but biallelic expressions in heart,liver,spleen,kidney,muscle and subcutaneous fat tissues,suggesting that the imprinting of Wif1 was tissue-specific in cattle.To analyze the possible role of DNA methylation in regulating Wif1 tissue-specific imprinting,the methylation status of 29 CpG sites in Wif1 gene promoter region was analyzed in lungs (monoallelic expressed tissues) and livers (biallelic expressed tissue) by bisulfite sequencing.The results showed that the methylation levels between the 2 parental strands exhibited no significant difference (P>0.05) neither in lung nor liver tissues.The difference of methylated rates of each CpG sites between 2 parental chains in lungs and livers was further analyzed,and 8 CpG sites exhibiting significant difference of methylation rate between parental chains were found in lungs compared to those in livers.Among them,CpG sites 1,2 and 6 were contained in the transcription factor biding site.Because the methylation changes of individual CpG sites can effect the binding of transcription factors,the methylation of 3 CpG sites in Wif1 promoter region may be involved in the regulation of tissue-specific imprinting of cattle Wif1 gene.

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